The callus multiplication rate of explant segments and callus segments induced from rhizome reached 150 times more than in the fresh weight of the callus, when cultivated in MS liquid medium containing 0.2–0.3 mg/l of 2.4-D at (25± 1) C and 30 rotations/min under light exposure for 28 days. In this study, although the control was cultured in medium without plant growth regulators, after 28 days of culture, multiplication rates were somewhat recognized. A particular interaction was observed between 0.2 mg/l 2.4-D and 0.2 mg/l BAP.