Based upon the calculation provided on the Isohop® website
(http://www.barthhaasgroup.com) for converting ml/hl of Isohop® to
BU, the range of BU originally tested was from 0.6 BU to 240 BU. Later,
this range was narrowed to 3–21 BU. This narrowed range of BU was
then tested with and without addition of ethanol to 3, 5, and 7% v/v.
The plates were sealed with parafilm and incubated for 48 h in a
candle jar at 30 °C.