To indicate the total water content in FSC and BAT collagen, sample masses measured at 293 K, 383 K and 473 K were used. Heating to 383 K led to a mass loss of 8% for FSC and, a mass loss of 13% for BAT collagen. When heated to 473 K, the mass loss was 17% for FSC and 18% for BAT collagen. This means that BAT collagen is more hydrated. According to [17,18], when measurements are performed for solid collagen in the temperature range of 290–380 K, the denaturation process will not be observed. Thus, the occurring peak can be explained as the result of the free water release process.