The total soluble flavonoid content was estimated by aluminium chloride colorimetric method15 for
aqueous, methanolic and ethanolic extracts of A. paniculata and T. cordifolia. 0.5 mL of stock solution (1
g/mL) of the extract, 1.5 mL methanol and 0.1 mL potassium acetate (1M) was added to reaction test tubes
and volume was made up to 5 mL with distilled water. After incubation at room temperature for 30 min, the
absorbance of the reaction mixture was measured at 415 nm and compared with quercetin, a positive control.