Loop full from all bacterial cultures were inoculated into MHB
then incubated at 37 C for 12 h to make inoculation cultures.
Oppositely, P. expansum and R. stolonifer spores were inoculated on
sabouraud agar and incubated at 28 C for 5 d. The fungal spore
suspensions were prepared by washing the 5-old day’s cultures by
10 mL tween 80 solution 1 mL L1 using glass rod to make a stock
suspension solution and count using Thoma’s cell with light
microscopy.
Olive oil processing residues preparation and extraction