Fig. 2. Assessment of RT-PCR sensitivity for RVs-D detection. Lanes 1–7, 10-fold serial dilutions of cleansed recombinant colonies containing the VP6 fragment of RVs-D, with initial concentration of 50 ng/L (lane 1). The lowest dilution yielding virus detection was 10−5(0.5 pg; lane 6); lane 8, 123-bp DNA marker from Invitrogen