Aedes albopictus larvae and pupae were collected
round the year from all artificial containers
and natural habitats from Faisalabad
(31°21′52″N 72°59′40″E) and brought to the
mosquito rearing room in Biosystematics lab
of department of Agri. Entomology, University
of Agriculture Faisalabad, reared in steel
trays of 3 inch depth and fed on TetraMin®
(fish food) Akram et al. (2010).