The total phenolics were conventionally quantified colorimetrically
using a modified Folin–Ciocalteu method (Singleton, Orthofer, &
Lamuela-Raventos, 1999). The reaction mixture, consisting of 125 μl of
methanolic tomato extract (0.1 g/10 ml), 0.5 ml of distilled water and
125 μl of Folin–Ciocalteu reagent,was allowed to stand for 6 min before
adding 1.25 ml of a 7% sodiumcarbonate and final volume was adjusted
to 3mlwith distilled water. Absorbance wasmeasured at 760 nm, after
90min and a standard curvewas obtainedwith gallic acid (50–600 μM).