equipped with a fluorescence detector. Labeled N-glycans were separated by a linear gradient of 20–58% of 50 mM ammonium formiate pH 4.4 against acetonitrile over 152 min at a flowrate of 0.4 mL/min. Samples were injected in 80% acetonitrile.The fluorescence detection was carried out using an excitation wavelength of 330 nm and an emission wavelength of 420 nm[31]. The elution positions of the N-glycans were determined in glucose units (GU) by comparison with a standard dextranhydrolysate 2AB labeled (dextran ladder) [12]. The glycosylation was also analyzed by mass spectrometry (MALDI-MS) usingan Axima Performance mass spectrometer (Shimadzu Biotech,Japan).