Using the primers designed initially for conventional
PCR, several real-time quantitative PCR (qPCR) assays have been developed. These assays are very sensitive and
minimum amounts of nucleic acids can be amplified and
accurately quantified. The use of qPCR has helped in the
quantitative monitoring of natural bloom populations. The
technique enabled the identification of minor strains within
these communities. It also provided insights into the environmental
and physiological parameters that stimulate or
inhibit the growth of toxic cyanobacteria and the production
of their toxins. For those reasons, qPCR is appealing as
an early warning diagnostic technique.