The objective of this research is to investigate the effect of two extraction techniques
(maceration and reflux) and three extracting solvents (methanol, butanol and ethylacetate) on
the yield and antioxidant activity of Peperomia pellucida L. Kunth extracts. The result
showed that the methanolic extract by reflux method offered the highest extraction yield.
Moreover, among the different extraction solvents, ethylacetate extract had the highest total
phenolic contents. This correlated to the antioxidant activity evaluated by DPPH assay. The
reducing power of extracts expressed as mol FeSO4/g extract were in the range of 0.139-
1.164 mol FeSO4/g extract. Overall, higher extract yield, total phenolic contents and
antioxidant activity were obtained by reflux method.