SDS-PAGE analysis of myofibrillar proteins
Myofibrillar proteins were resolved by sodium dodecyl sulfatepolyacrylamide
gel electrophoresis (SDS-PAGE) in a gradient gel
8–18%. The run was performed in a continuous buffer system using
a Protean II xi vertical slab gel unit (Bio-Rad Laboratories, Hercules,
CA). Gels were stained with Coomassie blue G250. Destained gel images
were acquired by the Chemi Doc EQ system (Bio-Rad Laboratories,
HLongissimus dorsi (LD) muscle was removed from each half carcass
24 h post mortem; each muscle was divided into two sections
resulting in four samples for each animal.