2. Experimental
2.1. Packaging and storage
Wheat bread was produced according to standard commercial practices by a local bread manufacturer using wheat flour, extraction rate 70%, bakers’ yeast and water. In case of commercial samples, the following additives were used at a 1% total concentration in wheat flour as dough improving agents: ascorbic acid (E300), calcium carbonate (E170) and diacetyl-tartaric esters of fatty acids (E472e). Baked loaves were cooled down to room temperature for 60 min, transferred to the laboratory within 30 min and sliced 2 cm thick. Bread composition was determined using
official AOAC methods (protein: 8.0%, fat: 1.7%, starch: 47.9%, sugars: 1.8%, fiber: 2.7%, moisture: 39.0%).