2.4. Toxicity of MvRL to B. glabrata embryos and adults
Adult B. glabrata, not infected by S. mansoni, were reared in the
Departamento de Biofísica, Universidade Federal de Pernambuco
(Recife, Brazil). Adult snails were reared in plastic tanks (50
23
17 cm) containing
filtered and dechlorinated water (27.5
2.5 C)
with aeration provided by an aerator pump, and were fed with
lettuce leaves. Transparent plastic strips were placed in the tanks to
act as an oviposition substrate.