Wine and grapeseed oil are both natural products. Red wines especially have a high content in natural antioxidants such as flavanoids, natural colourants such as anthocyanins and natural aroma compounds such as terpenes and esters [1] and [2]. Grape seed oil on the other hand is considered to be a dietary oil of high quality with a high concentration of unsaturated linoleic acid, vitamin E and phytosterols [3] and [4]. In this study, an attempt was made to prepare oil-in-water cosmetic emulsions by adding white or red wine in the aqueous phase and using grape seed oil as the oil phase in order to examine their stability with time and exploit their beneficial constituents. The study was complemented by using the natural resin from the mastic tree of Pistacia lentiscus var. Chia as additive to examine its impact on the organoleptic and rheological characteristics of the emulsions. The mastic resin was chosen as a natural additive of Hellenic origin with healing and antibacterial properties