Biodegradation of Distillation Products.
The bacterial strains from overnight culture (104–105 cells/ml) were transferred to 100 ml of sterile mineral medium (MM) with 0.1% (v/v) of refined petroleum products as a carbon and energy source. Removal of petroleum products was done after 20 days of incubation time. Sterile controls were prepared to evaluate hydrocarbon evaporation. The experiment was done in triplicate.