Effects of cryopreservation on sperm motility, semen amidase activity, and Western blot analysis profiles recognized by anti-acrosin I antibodies
3.5.1. Sperm motility characteristics and amidase activity of spermatozoa
Cryopreservation significantly decreased the per- centages of sperm motility and all sperm motility char- acteristics (Table 2). The dilution of semen with DMA resulted in decreased extracted amidase activity (P 0.05). However, this activity after cryopreservation did not change significantly (Fig. 8A). No differences were found in the amidase activity of sperm suspension in fresh and frozen-thawed semen (Fig. 8B). Extracted amidase activity was not correlated with amidase ac- tivity before and after cryopreservation (r values for
Table 2
Effect of cryopreservation on sperm motility characteristics.
Computer-assisted Cryopreservation
fresh semen, fresh with the addition of DMA, and after freezing-thawing were 0.35, 0.67, and 0.52, respec- tively; P 0.05).