primary PCR with previously published primers for the merozoite antigen-1 (EMA-1) gene and nPCR with designed
primers for EMA-1. For the nPCR, forward primer EMA-F (5′-CGTCGAGAAGGTCAAGGAAG-3′) and reverse primer EMA-R (5′-
GACTCGAAGCCCTTGAACAG-3′) were used. Primers were designed using OligoPerfect Designer