down the length of the column in a sequence determined
by mass relationships and absorption coefficients [3].
Despite the tremendous potential impact of his discovery,
chromatography was not revived until 1931, under
the stimulus of widespread research on the separation of
carotenoids from several natural sources by adsorption analysis
on fibrous alumina [4]. Since the cited work was published,
chromatography application has been extended over
practically all areas of chemistry. In polymer science, chromatography
was used for the first time by Moore in 1963
to determine the MWD of polymers [5].