Tailored electrochemical sensor (GNRred-based) shows an impressive analytical performance for simultaneous electrochemical sensing of AA, LD and UA. These synthesized GNRred that contain remaining oxygen moieties and high content of carbons with sp2 hybridization exhibited a marked electrocatalysis towards the simultaneously determination of these target molecules. The excellent electrocatalysis exhibited by this GNR-red electrochemical sensor allowed a fast and reliable UA assessment in urine samples opening novel avenues for the construction of novel GNR-based electrochemical sensors for POC testing applications.