, the effect of pH on t-CA
formation in 50.0 mM Tris-HCl buffer was further investigated and the results are shown
in Fig. 3. The pH-enzyme activity curve was not a typical bell-shaped curve but had a maximum at pH 9.0. It is pertinent to mention here that almost all previous studies on the
effect of pH on PAL activity have reported that the optimum pH is in the range of 8.5 –
9.0