• Enriches mutant DNA in a mutant/wild-type mixture
by preferential amplification in PCR
• Based on exploitation of the critical temperature,
Tc, at which mutation-containing DNA is
preferentially melted over wild-type– Tc determined empirically by real-time or gradient PCR
• Preference in synthesis is repeated over many PCR cycles
• The greater the Δ(Tm-Tc) the greater the enrichment of mutant DNA