Results and discussion
Manual Annotation
Manual annotation and curation of the CYPs in Daphnia
pulex v1.1 draft genome sequence assembly (September,
2006) produced 73 full length P450s and three pseudogenes.
In addition, there are two ESTs in the CYP4 clan
that were not found in the D. pulex genome of the chosen
parthenogenic individual, but partial sequences
(Cyp4C32, Cyp4AN1) were observed previously in specific
D. pulex ecotypes [15]. Overall, this number is similar
to other CYP genomes (Additional file 1), but slightly
lower than the typical invertebrate or insect with the
exception of honeybee