Furthermore, for the 31 eyes examined by observer B,
the contralateral eyes were also fixed and embedded in
epoxy resin, and morphological parameters were measured
from histological sections. The morphological parameters
obtained from 27 sections of contralateral eyes were
recorded, together with f0 obtained from the corresponding
ipsilateral eyes (Table 2), data for the remaining four eyes
were omitted because of their oblique section planes. The
apparent refractive index n1 was estimated to be
1.77 ± 0.05. A lens–F0 distance was also calculated from
f0 and n1, and was determined to be 103 ± 17 lm for wholemount
observations and 87 ± 15 lm from sections (Table
2). The calculated distances were larger than those measured
by direct observation of the beam through the corneal
lens, but much closer to them than values calculated
by substituting 1.6 for an apparent refractive index n1.