Cattle and Buffalo husbandry practice have undergone progressive improvement over the years. Artificial insemination (AI)
has become the most important single technique for the improvement of the livestock resources in the tropical developing
countries for the development of highbred cattle. Frozen semen (-196ºC) from selected exotic bulls is used for insemination
purposes in Africa and Asia with variable results. Some variations in number of services per conception in different lactations
have been reported to be due to difference in fertility ofexotic frozen semen. In this paper, an overview of the
cryopreservation of cattle and buffalo bull semen is discussed with the view of improving the current AI practices in the
developing world.