measurements in different species described here, it can be seen that ratios obtained for
one species cannot be used for any other species.
In summary, several enzymes should be chosen with differing sensitivity and
specificity for the detection of major organ toxicity. The choice of these enzymes should
reflect their different tissue and intracellular locations. In more detailed studies, sampling
times should be used with some reference to enzyme production and clearance related to
the toxic insult. The use of such approaches is exemplified in the following chapters on
nephrotoxicity, hepatotoxicity and cardiotoxicity. Whether through lack of technical
simplicity or suitability for use with automated laboratory analysers, the number of
enzymes measured in regulatory studies has not increased dramatically over the previous
two decades.