Hence, 10% w/v substrate load was considered to be the optimum for enzyme hydrolysis of cellulose with reducing sugar yield of 21.76 ± 0.43% w/w (Fig. 2e). Combination of Stargen® 002 and Accellerase® 1500 under optimized conditions could produce 41.2 ± 0.12 g/l of total reducing sugars synergistically (Fig. 3).