Table 1
Ingredients of the high-fat diet.
Ingredients Amounts, g/kg diet
Powder skim milka 380
Soya oilf 60
Butterf b 30
Maize starch 300
Sucrose 155
CaCO3 20
Na2PO4 20
KCl 5
NaCl 5
Egg yolkc 17
Mineral mixtured 2.7
Vitamin mixturee 5.5
a As source of protein (Inesfood- Tunisia).
b As source of supplemented fat.
c As source of choline and L-cysteine.
d Mineral mixture (grams per kilogram dry weight of diet):
MgSO4$7H2O ¼ 1.85; ZnSO4$7H2O ¼ 0.50; MnSO4$4H2O ¼ 0.15;
CuSO4$5H2O ¼ 0.020; KIO3 ¼ 0.0015; FeSO4$7H2O ¼ 0.200.
e Vitamin mixture (per kilogram dry weight of diet): synthetic
vitamin A concentrate ¼ 6.500 IU; cholecalciferol ¼ 1.300 IU; atocopherol
acetate ¼ 2.6 mg; pyridoxine hydrochloride ¼ 2.6 mg;
thiamin hydrochloride ¼ 2.6 mg; riboflavin sodium
phosphate ¼ 1.95 mg; nicotinamide ¼ 13 mg; ascorbic acid ¼ 65 mg;
dexpanthenol ¼ 5.2 mg and finely powdered sucrose to make 5 g.
f Total lipids composition of HFD (%) 8.85 ± 0.6. Fatty acids from total
lipid content were 30.4% saturated fatty acids, mainly palmitic acid
(C16: 0) and myristic acid (C14:0), 36.4% monounsaturated fatty acids,
mainly oleic acid (C18:1 n-9), 33.3% polyunsaturated fatty acids, mainly
linoleic acid (C18:2 n-6), linolenic acid (C18:3) and Docosahexaenoic
acid (C22:6 n-3).