and SAN Extra L (glucoamylase from Aspergillus niger with declared activity of 400 glucoamylase uits per gram) and, mentioned above, Neutrase 0.8 L. Commercial active dry yeast Saccharomyces cerevisiae Ethanol Red (Fermentis, France) was used as production inoculum, it contained over 20 Â 109 of living cells per gram as declared by the manufacturer. Prior to inoculation the yeast were rehydrated in sterile distilled water (1 g of yeast preparation in 10 mL of water) at 30 °C for 0.5 h with regular agitation.