To enrich P. redivivus, 2 g of Superselco® (Inve Aquaculture) was added to 15 mL of freshwater and mixed to create an emulsion that was added to a conical 250 mL well-aerated flask containing the nematodes. After 5 h of exposure to Superselco®, the nematodes were collected in a fine mesh, rinsed with water and added to the fish tanks. be fed to the fish.