First, the bran was treated with 5% w/v KOH solution (ratio of 1:20) under mechanical stirring at room temperature for 14 h, which solubilized the pectins and the hemicelluloses. The insoluble residue was delignified with 1% w/v NaC102 at pH 5.0 (adjusted with 10% v/v acetic acid) and 70 °c for 1 h.