2.5. Hardening and acclimatization
Survival rate (%) was recorded after 60 days from transfer to greenhouse
conditions. Plantlets were initially covered with a polythene
sheet to maintain relative humidity (90%). The hardened plants were
regularly sprayed with 1/10th liquid MS medium. The number of surviving
plants was recorded after 12 weeks of transfer. All of the surviving
acclimatized plants were transferred to a greenhouse. The recorded
data were subjected to analysis of variance using SAS and means were
compared using Duncan's multiple range test (Duncan, 1955).