To produce a vaccine with adjuvant, the vaccine was emulsi- fied in an equal volume of 2% -(1,3/1,6)–glucan from Saccharomyces cerevisiae (MacroGard® AquaSol, Biorigin Scandinavia AS, Norway) to achieve final vaccine concen- tration of 1x1010 CFU/ml and stored at 4°C until used