In summary, we have described a relatively simple FIA system
to detect serum glucose in a wide linear range of 0.1–50 mM
using a fabricated glucose sensor that was modified with composite
films to prevent interferences and to entrap enzyme. Animal
blood serum was directly injected and assayed in the FIA system.
Good reproducibility and analytical recovery were achieved using
the proposed method. The main advantages of this new FIA system
are the wide linear range for detection and the potential to
have a high throughput assay system, which can be referred as the
simulation of the flowing physiological system when compared to
the measurement of glucose in blood serum or whole blood using
the previously reported method [22,23]. In addition, electrochemical
arrangement used here for FIA measurements of glucose could
also be employed as a simplified HPLC method [24], and could
be applied for detection of serum glucose for normal people and
diabetics under optimized conditions.