The extraction solvent in DLLME has to meet several characteristics,
including low volatility, low water solubility, higher density
than water, and high capability for extracting target compound.
Based on these requirements, carbon tetrachloride, trichloromethane,
and dichloromethane were chosen as extraction solvent.
Therefore, studies were conducted using different volumes of
these extraction solvents containing a constant amount of Aliquat
336. The cloudy solution and two-phase system appeared by
applying carbon tetrachloride and trichloromethane whereas this
event was not observed using dichloromethane. However, carbon
tetrachloride was chosen, because in this case the highest and
more reproducible signal was observed and smaller volume of
extraction solvent was required in the injection step