Insect bioassay
To test the efficiency of transgenic plants’ resistance against in-sect pests, insect bioassays were conducted twice using the larvae
of tobacco budworm (Heliothis virescens) kindly provided . T4 and T5 gen-eration of transgenic plants and non-transgenic control plants
were grown under contained condition and used for feeding exper-iments. In the first experiment, seven each transgenic and non-transgenic plants were used, while for the second experiment, 10
plants each were used. Five larvae were inoculated on each plant