The isolates were tested for pathogenicity using detached leaves in the laboratory. A sterilized filter paper was placed in a sterilized 9cm-diameter petri disc. Tea leaf was made wound by sterilized needle and then placed on the filter paper in the petri dish. A 0.5 cm diameter sterilized cork borer was used to remove agar plugs from the actively growing edge of cultures of the pathogenic fungus onto the wounded position of tea leaf surface. The filter paper in the plate was moistened by sterilized distilled water. The non-inoculated leaves treated with 0.5cm sterilized agar plug served as controls. The experiment was done using Completely Randomized Design (CRD) with repeated four times and incubated at room temperature (27-30ºC) for 10 days.