A previous report
indicated that NNV (C-06-HNV-RI97) propagated in E-11
result in 95% cumulative mortality when Atlantic cod (7 g
of average weight) were given 107 TCID50 of that per fish
(Krasnov et al., 2013). In the present study, 63% of
cumulative mortality was observed when seven-band
grouper (63–88 g) was challenge with 109 TCID50 of
RGNNV, which was propagated in E-11 cells (PBS group
in Fig. 3).