The study aimed firstly to determine whether, and the extent to which, EI is associated with affect at work and job satisfaction. Based on recent findings (Lopes et al., 2006; Sy et al., 2006) we expected that self-reported EI abilities will be associated with positive affect and job satisfaction and inversely related to negative affect at work. Moreover, we wanted to examine which EI dimensions may be related to positive and negative affect and job satisfaction. Secondly, we aimed to test whether, and the extent to which, positive and negative affect at work mediate EI effects on job satisfaction. Based on Affective Events Theory, we expected that work affect would at least partially mediate EI effects on job satisfaction; we did not have any hypotheses with regards to the relative strength of positive and negative affect in this relationship. We applied a Structural Equations Modelling approach in order to clearly distinguish the mediatory power of positive and negative affect while controlling for common variance between positive and negative affect.