Fig. 6. Effect of porcine placenta extract on the expression of pro-inflammatory cytokines. (A) Single cell suspended total draining lymph node cells and (B) CD4+ T cells were stimulated for 2 h with PMA and ionomycin. Total RNAs were isolated from each sample and reverse transcribed to obtain cDNA. Relative expression level of the cytokines in the experimental group was compared with control group by quantitative real-time PCR. Results are expressed as relative expression levels by taking the expression of the gene in vehicle group as 100%. Error bars indicates SD. One (*) or two (**) indicate p < 0.05 or p < 0.005, respectively. Data are representative of three independent experiments.