2.4. BC production and culture conditions
2.4.1. Pre-inocula preparation
The pre-inocula were prepared by growing the microorganisms
at 30 C during 48 h, in static condition, in HS liquid
medium (20 g L1 glucose, 5 g L1 peptone, 5 g L1 yeast
extract, 2.7 g L1 Na2HPO4, 1.15 g L1 citric acid, agar 15 g L1,
pH 5) before inoculation of 5 mL into 45 mL of liquid production
medium in 250 mL Erlenmeyer flasks.