NUCLEIC ACID
Virions contain a single molecule of linear, negative sense ssRNA (Mr 4.2 106–4.6 106, about
11–15 kb in size). The RNA represents about 1–2% of virion weight. The RNA has a 3-terminal free
hydroxyl group and a 5-triphosphate and is not polyadenylated. The ends have inverted comple-
mentary sequences with transcription and replication initiation signals. Defective RNAs, usually
significantly shorter than full-length RNA (less than half size), may be identified in RNA recov-
ered from virus populations. They are usually negative sense; however, hairpin RNA forms are also
found. Defective RNAs replicate only in the presence of homologous and, occasionally, certain het-
erologous helper rhabdoviruses which provide the functional genes. Full-length positive sense RNA,
which is an intermediate during the replication process, may constitute up to 5% of a viral RNA pop-
ulation. Like the full-length negative sense RNA genome, it is permanently bound to N protein.
PROTEINS
NUCLEIC ACID
Virions contain a single molecule of linear, negative sense ssRNA (Mr 4.2 106–4.6 106, about
11–15 kb in size). The RNA represents about 1–2% of virion weight. The RNA has a 3-terminal free
hydroxyl group and a 5-triphosphate and is not polyadenylated. The ends have inverted comple-
mentary sequences with transcription and replication initiation signals. Defective RNAs, usually
significantly shorter than full-length RNA (less than half size), may be identified in RNA recov-
ered from virus populations. They are usually negative sense; however, hairpin RNA forms are also
found. Defective RNAs replicate only in the presence of homologous and, occasionally, certain het-
erologous helper rhabdoviruses which provide the functional genes. Full-length positive sense RNA,
which is an intermediate during the replication process, may constitute up to 5% of a viral RNA pop-
ulation. Like the full-length negative sense RNA genome, it is permanently bound to N protein.
PROTEINS
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