The C. longa plant and tubers are shown in Figure 1A and B. The C. longa tubers were washed to remove the adhering mud particles and possible impurities. Later they were dried under sunlight for a week to completely remove the moisture. The tubers were cut into small pieces, powdered in a mixer, and then sieved using a 20-mesh sieve to get uniform size range. The final sieved powder was used for all further studies (Figure 1C). For the production of extract, 0.1 g of C. longa tuber powder was added to a 100 mL Erlenmeyer flask with 20 mL sterile distilled water and then mixed for 4 hours at room temperature.