More than 70 out of the total 180 isolates shared a 100% sequence homology of the 16S rRNA gene with Micromonospora aquatica. These were isolated from sponges and sediment samples of Puerto Rico. Ten isolates from sponges and sediments were selected and upscaled for characterization of their chemical profiles. Following fractionation of the crude extracts from these Micromonospora aquatica strains, fractions were subjected to LC-UV-MS analysis. From these data, a clear distinction between sponge associated M. aquatica to those isolated from sediments was observed. The fractions from the same solvent system between different sediment associated M.aquatica showed similar UV absorbance values and molecular weights (Table 20). Compounds with molecular weights of 585 m/z, 601 m/z were seen in 6 out of the 10 sediment M. aquatica isolates analyzed. All UV absorbance values for the individual actinomycetes showed a common λmax of 220 nm with minor absorbance at other wavelenghts. Moreover, the physical appearance of sediment associated M. aquatica was similar in all isolates. The growing morphology of these strains consisted of orange colonies that overgrow the agar plates in solid media and in liquid media produce a brown cluster of small cells (Fig. 29). No diffusible pigments were observed in their culture broths.