MATERIALS AND METHODS
Plant materials
The single node explants containing lateral buds of actively fieldgrown
‘Miniature’ rose were used for multiplication experiments.
They were cut in 5-6 cm length segments and surface-disinfested
using 70% ethanol for 20 s and then immersed in laundry bleach
(5.25% Hypochorite Sodium) solution of commercial containing 2
drops of Tween-20 emulsifier to aid wetting for 20 min (Nak-Udom
et al., 2009). The sterilized explants were washed 2-3 times with