Statistical methods e macrofungi on all kinds of wood A variety of summary statistics and statistical procedures was
used to examine the macrofungi on each of the four woody substrates, viz. CWD, ODW, stags and living trees. For species
richness, this included the use of species accumulation curves calculated using the MaoeTau estimator available in the
package EstimateS (Colwell 2005). This theoretical estimator for sample-based rarefaction removes seasonality and random variation among visits, effectively producing a randomized species accumulation curve (Colwell et al. 2004). For species assemblages, non-metric multidimensional scaling (nMDS), as implemented in PRIMER v6 (Clarke & Gorley 2006), was used to explore whether there were indications of differences among substrata types without imposing constraints or hypotheses.
Similarity (resemblance) was defined employing the widely
used BrayeCurtis measure (Bray & Curtis 1957), the database
being the‘species by visits’matrixwhere subplotswere ignored.
In addition, the possible presence of indicator species (sensu
Dufreˆne & Legendre 1997) to characterize each of the four
substratawas examined using the packagePC-ORDVersion4.10
(McCune & Mefford 1999).