In addition, a slight decrease in the myosin heavy chain content
(MHC, 200 kDa, Fig. 4B) with storage time was observed. Benjakul,
Seymour, Morrissey, and An (1997) reported that the MHC content
of Pacific whiting decreased by almost 60% after fish were stored in
ice for 8 days. An, Weerasinghe, Seymour, and Morrissey (1994) reported
that the high activity of cathepsin L and B resulted in the
proteolytic degradation of Pacific whiting during ice storage. The
electrophoretic patterns also showed a decrease in the intensity
of low-molecular-weight protein bands with storage time, such
as that at 35–55 kDa. Yongsawatdigul and Park (2002) reported a
slight decrease in MHC content and an MHC intensity at 0 day of
storage that was comparable with that of samples stored for
9 days, which is consistent with the results of our study