The present study is focused on the development of a cultivation-independent molecular approach for specific detection of bioluminescent bacteria within microbial communities by direct amplification of luxA gene from environmental DNA
A new set of primers ,specifically targeting free-living bioluminescent bacteria , was designed on the base of luxA sequences available from the public database
Meso - and bathypelagic seawater samples were collected from two stations in TyrrhenianSea at the depths of 500 and 2750 m.
The same seawater samples also were used to isolate bioluminescent bacteria that were further subjected to luxA and 16S rRNA gene sequencing