Injection volume for samples was set to 1 μL. The needle was washed with 50% methanol, 50% acetonitrile, and 0.01% formic acid at the flush port (3×) after each injection to minimize carry over.
Injection volume for samples was set to 1 μL. The needle was washed with 50% methanol, 50% acetonitrile, and 0.01% formic acid at the flush port (3×) after each injection to minimize carry over.