0.5 ml of PM was introduced onto a 2.5 cm. x 2.5 cm. occlusive gauze patch and placed on the
shaven skin and secured to the skin of the test animal by transpore adhesive tape. The entire
trunk of the animal, each was wrapped with elastic cloth to avoid dislocation of the patch for
6 hrs exposure period. At the end of the exposure period, all patches were removed and gently wiped the treated skin with a water moistened cotton wool to remove any residual of test sample.